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cho-k1 membranes expressing human cxcr2 receptors  (BioSignal Group)

 
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    BioSignal Group cho-k1 membranes expressing human cxcr2 receptors
    Cho K1 Membranes Expressing Human Cxcr2 Receptors, supplied by BioSignal Group, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cho-k1 membranes expressing human cxcr2 receptors/product/BioSignal Group
    Average 90 stars, based on 1 article reviews
    cho-k1 membranes expressing human cxcr2 receptors - by Bioz Stars, 2026-03
    90/100 stars

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    Mechanistic analysis of chondrocyte cell growth and migration induced by MSC-EVs. A RT-qPCR analysis of CXCL5 and CXCL6 expression in chondrocytes treated with MSC-EVs (n = 6). The data shown represent the fold change relative to the control group. AKT and ERK were inhibited using 7 μM LY290042 and 20 μM PD98059, respectively. B Western blotting following AKT and ERK inhibition in response to MSC-EV treatment. C Chondrocytes were treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist and then stained with crystal violet. D CCK-8 cell growth assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. Absorbance corresponds to cell number. Measurements were repeated five times, and the average value was calculated (n = 6). E Transwell migration assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. F Chondrocytes migrating to the lower surface were counted in five randomly selected fields (n = 6). Data are the mean ± SD. * P < 0.05 according to the Mann-Whitney U test

    Journal: Stem Cell Research & Therapy

    Article Title: Extracellular vesicles derived from mesenchymal stromal cells mediate endogenous cell growth and migration via the CXCL5 and CXCL6/CXCR2 axes and repair menisci

    doi: 10.1186/s13287-021-02481-9

    Figure Lengend Snippet: Mechanistic analysis of chondrocyte cell growth and migration induced by MSC-EVs. A RT-qPCR analysis of CXCL5 and CXCL6 expression in chondrocytes treated with MSC-EVs (n = 6). The data shown represent the fold change relative to the control group. AKT and ERK were inhibited using 7 μM LY290042 and 20 μM PD98059, respectively. B Western blotting following AKT and ERK inhibition in response to MSC-EV treatment. C Chondrocytes were treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist and then stained with crystal violet. D CCK-8 cell growth assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. Absorbance corresponds to cell number. Measurements were repeated five times, and the average value was calculated (n = 6). E Transwell migration assay of chondrocytes treated with MSC-EVs and an ERK inhibitor or a CXCR2 antagonist. F Chondrocytes migrating to the lower surface were counted in five randomly selected fields (n = 6). Data are the mean ± SD. * P < 0.05 according to the Mann-Whitney U test

    Article Snippet: A human chemokine CXCR2 receptor antagonist (SB265610 [#1559]; Axon Medchem, Groningen, The Netherlands), PI3K signaling inhibitor (LY290042 [#129-04861]; Wako Pure Chemical Industries Ltd.), and Erk1/Erk2 signaling inhibitor (PD98059 [#9900]; Cell Signaling Technology) were used to determine the involvement of the CXCR2, AKT, and ERK pathways on the chondrocytes treated with MSC-EVs.

    Techniques: Migration, Quantitative RT-PCR, Expressing, Control, Western Blot, Inhibition, Staining, CCK-8 Assay, Growth Assay, Transwell Migration Assay, MANN-WHITNEY